Age-related genetic changes in the blood associated with poor cancer prognosis microbiologystudy

Researchers from the Francis Crick Institute, UCL, Gustave Roussy and Memorial Sloan Kettering Cancer Center (MSK), have discovered that expansion of mutant blood cells, a phenomenon linked to aging, can be found in cancerous tumors, and this is associated with worse outcomes for patients.

Understanding the biological interface of age-related genetic changes and diseases of aging, such as cancer and cardiovascular disease, is important to develop preventative therapies for a growing proportion of the population.

Clonal hematopoiesis of indeterminate potential (CHIP) is a condition where blood stem cells accumulate mutations over time, influenced by both aging and external environmental factors. CHIP has already been shown to be associated with risk of age-related disorders, such as cardiovascular disease, but the impact of these genetic changes on solid cancer evolution hasn’t been thoroughly investigated.

The work, published today in the New England Journal of Medicine, is a detailed study of the link between CHIP and cancer, in over 400 patients with lung cancer as part of the TRACERx and PEACE studies, and 49,000 patients with different types of cancer from MSK.

CHIP and cancer prognosis

An initial examination of blood samples allowed the research team to determine which patients had CHIP mutations in their blood. When matched with clinical data, the scientists observed that these mutations were associated with patients living for a shorter period of time, regardless of their age or the stage at which the cancer was diagnosed.

The researchers then went on to study patients with CHIP in more detail and determine whether the specific mutations were also present in their lung tumors because of blood cell infiltration. This was found to be true in 42% of patients with CHIP and they called this phenomenon tumor infiltrating clonal hematopoiesis (TI-CH). The team found that it was TI-CH, not CHIP alone, that was associated with the greater risk of cancer relapse and cancer death.

This finding was supported by samples from the PEACE study, a postmortem investigation of areas where cancer has spread, the main cause of cancer death. The team found that metastatic tumors at these sites often contained TI-CH mutations.

Not all mutations are equal

To inspect the link between TI-CH and poor patient outcomes, the scientists went on to look at the composition of cells in the lung tumors. They found that patients with TI-CH had an expansion of myeloid cells, a type of immune cell. These cells are an important part of the tumor microenvironment and unlike some immune cells that are primed to recognize and fight cancer, myeloid cells have been shown to regulate inflammation and can support tumor progression and spread.

The researchers also discovered that when mutations affected a gene called TET2, which is an important regulator of blood cell production, across thousands of individuals, the TET2 mutant blood cells were more likely to infiltrate the tumor. When analyzing hundreds of single cells from the tumors of two patients with TI-CH, they confirmed that TET2 mutations were mostly present in myeloid cells but not in other immune cell types.

The team then collaborated with blood cancer and CHIP experts in a Crick lab led by Dominique Bonnet, to study the impact of TET2 mutations experimentally. Together they grew organoids, mini lung tumors, with TET2 mutant myeloid cells. They showed that TET2 mutant myeloid cells remodeled the tumor microenvironment and accelerated tumor organoid growth.

Looking beyond lung cancer

Finally, in collaboration with researchers at Memorial Sloan Kettering Cancer Center in the US, the team validated their findings using a much larger data set of over 49,000 patients with different types of cancer. Overall, the presence of TI-CH was an independent predictor of shorter survival. But the presence of CHIP and TI-CH varied between cancer types. Researchers found these mutations were more common in cancers known to be harder to treat like lung cancer, head and neck cancer and pancreatic cancer.

The next steps for this work will be to confirm that CHIP directly contributes to cancer outcomes and then detail the exact mechanism by which CHIP is functionally implicated in the development of aggressive cancers.

Oriol Pich, Postdoctoral Project Research Scientist in the Crick’s Cancer Evolution and Genome Instability Laboratory, said, “Our results show that blood cells carrying age-related mutations can infiltrate tumors and impact cancer evolution, leading to worse outcomes for patients.

“This is important because CHIP is a natural phenomenon of aging that is common in patients with cancer.”

Charlie Swanton, Deputy Clinical Director at the Crick, Chief Clinician at Cancer Research UK and Chief Investigator for TRACERx, said, “This is the first time that we’ve been able to see at scale, the interaction of two different types of ‘clonal proliferations,’ age-related CHIP and cancer, providing insight into how aging might impact cancer risk.

“As we start to piece together the picture of the most important mutations which evolve during the aging process in cells from the bone marrow, and the impact they have in disease, we hope we can start to identify opportunities for intervention and maybe even prevention of some age-related cancers.”