Southern Blotting: principle, procedure and application
Southern Blotting: Principle A Southern Blotting is a method used in molecular biology for detection of a specific DNA sequence […]
Genetics
What is Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on
Restriction Enzymes and Site-Specific DNA Cleavage
Restriction Enzymes: Procedures for chemical isolation of DNA, such as those developed by Avery, MacLeod, and McCarty, usually lead to
The DNA Microarray: An Overview
In the ever-evolving field of genetics and molecular biology, scientists are constantly searching for innovative ways to unlock the mysteries
What is DNA Cloning
DNA cloning. Also referred to as gene cloning or molecular cloning, DNA cloning is a method used to produce multiple
Plasmid: Characteristics, Types, Functions, and Host Range
Plasmid Properties/Characteristics of bacterial plasmids: Physical properties: Replication: Curing: Incompatibility: Transferability: Recombinations: Mobilisation: Types of plasmid: 1. R-plasmid (R-factor): 2.
Artificial Chromosomes: Exploring Recombinant DNA Technology
Introduction Genetic manipulation has proven to be a relatively straightforward process in prokaryotes and lower eukaryotes. However, when it comes
The Polymerase Chain Reaction: Revolutionizing Biotechnology
The Polymerase Chain Reaction (PCR) is a groundbreaking technique that revolutionized the field of biotechnology. Developed by Kary Mullis between
DNA Repair Mechanism
Introduction In the realm of extreme biological capabilities, one organism stands out for its extraordinary ability to repair its genome
Detection and Isolation of Mutants
Mutation plays a crucial role in genetic research and the study of various organisms. Detecting and isolating mutants is an