In vivo CRISPR screen reveals pancreatic cancer tumors suppressors. A Experimental design of the in vivo PDAC CRISPR screen, showing gene selection from long-tail mutations, pancreatic injection of AAV libraries and tumor sequencing. B Tumor-free survival of Pdx1-Cre;LSL-KrasG12D;LSL-Cas9-GFP mice transduced with a sgRNA library targeting putative pancreatic cancer genes (n = 23) or a control sgRNA library […]
Credit: Unsplash/CC0 Public Domain Women treated for ovarian cancer caused by an inherited faulty gene have a lower risk of developing breast cancer following that treatment, new research from Manchester University NHS Foundation Trust (MFT) and The University of Manchester has revealed. MFT researchers say it is likely this is because of the platinum-based chemotherapy
Comprehensive integrated multimodal human kidney single-cell atlas. a, Overview of the multimodal analysis. b, UMAP of 338,565 cells/nuclei in an integrated human kidney snRNA-seq/scRNA-seq and snATAC–seq data generated in this study. c, UMAP of 588,425 integrated human kidney snRNA-seq/scRNA-seq and snATAC–seq data from the present study and KPMP. Credit: Nature Genetics (2024). DOI: 10.1038/s41588-024-01802-x Researchers
Design and test of split intein constructs to assemble FL-dystrophin. Credit: Nature Communications (2024). DOI: 10.1038/s41467-024-50569-6 Indiana University School of Medicine researchers have made a significant breakthrough in developing a new gene therapy approach that restores full-length dystrophin protein, which could lead to new treatments for people with Duchenne muscular dystrophy (DMD). The study, published
Graphical abstract. Credit: iScience (2024). DOI: 10.1016/j.isci.2024.110395 Millions of adults have atrial fibrillation—an irregular beating of the upper chambers of the heart that yields increased risk of heart failure, stroke and death. Many genetic mutations in the developing fetus can lead to adult atrial fibrillation, including mutations that shorten the massive protein titin in cardiac
Credit: Pixabay/CC0 Public Domain As a species, humans like to think that we are fully in control of our decisions and behavior. But just below the surface, forces beyond our conscious control influence how we think and behave: our genes. Since the 1950s, scientists have been studying the influences genes have on human health. This
Scientists have succeeded in pinpointing the neurons within a female fruit fly’s brain that respond to signals from the male during mating. Male fruit flies transfer a substance called a sex-peptide during mating in the seminal fluid together with sperm. This sex pheromone influences the female fly’s behaviour so she will start to lay eggs
Cdk8 regulates mitochondrial morphology under physiological conditions. Credit: Nature Communications (2024). DOI: 10.1038/s41467-024-47623-8 Researchers at Simon Fraser University, in collaboration with a group from Baylor College of Medicine in Texas, have identified a gene that appears to reverse Parkinson’s disease symptoms in fruit flies. SFU’s Verheyen lab discovered that increasing the amount of the fruit
A little leg may reveal something big about how closely related insect species can drastically differ in body shape, according to a new study led by researchers at the University of Tokyo. The team used time-lapse microscopy to image the live cells of fruit flies and found a new structure, which forms and disappears in
Identification of misexpression events and characterization of misexpressed genes. Credit: The American Journal of Human Genetics (2024). DOI: 10.1016/j.ajhg.2024.06.017 Scientists have uncovered that “gene misbehavior”—where genes are active when they were expected to be switched off—is a surprisingly common phenomenon in the healthy human population. The team also identified several mechanisms behind these gene activity