High glucose induces DNA methyltransferase 1 dependent epigenetic reprogramming of the endothelial exosome proteome in type 2 diabetes microbiology

Abbreviations

ACD

Automatic Gain Control

AGEs

Advanced Glycation End Products

CAMP

Cyclic Adenosine Monophosphate

CD31

Cluster of Differentiation 31

CD63

Cluster of Differentiation 63

CD9

Cluster of Differentiation 9

CGMP

Cyclic Guanosine Monophosphate

DDA

Data-Dependent Acquisition

DNMT1

DNA Methyltransferase 1

EDTA

Ethylenediaminetetraacetic Acid

eNOS

Endothelial Nitric Oxide Synthase

EVs

Extracellular Vesicles

FTO

Fat Mass and Obesity-Associated Protein

HbA1c

Glycated haemoglobin

HCD

Higher Energy Collision Dissociation

HCT116

Colorectal Carcinoma Cell Line

HDLc

High-Density Lipoprotein Cholesterol

HUVECs

Human Umbilical Vein Endothelial Cells

ICAM1,

Intercellular Adhesion Molecule 1

KCNQ1,

Potassium Voltage-Gated Channel Subfamily Q Member 1

LC-MS/MS

Liquid Chromatography-Mass Spectrometry/Mass Spectrometry

MMPs

Matrix Metalloproteinases

MTOR

Mammalian Target of Rapamycin

PBS

Phosphate-Buffered Saline

PDX1,

Pancreatic and Duodenal Homeobox 1

PSMs

Peptide-Spectrum Matches

RKO

Human Colon Carcinoma Cell Line

ROS

Reactive Oxygen Species

SDS-PAGE

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

SiRNA

Small Interfering RNA

SMAD12,

Mothers Against Decapentaplegic Homolog 1 and 2

SOCS3,

Suppressor of Cytokine Signaling 3

T2DM

Type 2 Diabetes Mellitus

TCF7L2

Transcription Factor 7-Like 2

TEABC

triethylammonium bicarbonate

TGFβ-1

Transforming Growth Factor Beta 1

TNF-alpha

Tumor Necrosis Factor Alpha

TXNIP,

Thioredoxin-Interacting Protein

VEGF,

Vascular Endothelial Growth Factor

VSMCs

Vascular Smooth Muscle Cells

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