Single-cell RNA sequencing reveals heterogeneity in fibrotic scars after spinal cord injury microbiologystudy

Myofibroblasts generate fibrotic scars after spinal cord injury (SCI). This is typically regarded as an impediment to nerve regeneration. Understanding the heterogeneous characteristics of fibrotic scars might help to develop strategies for remodeling fibrotic scars after SCI. However, the composition, origin and function of fibrotic scars have been a subject of ongoing debate in the field.

A recent study led by Profs. Dai Jianwu and Zhao Yannan from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences employed a combination of lineage tracing and single-cell RNA sequencing (scRNA-seq) to demonstrate the heterogeneous distribution, source, and function of meningeal fibroblasts and perivascular fibroblasts in fibrotic scars.

Their research is published in the journal Nature Communications.

Previous studies have reported that in non-penetrating spinal cord injuries, myofibroblasts primarily originate from perivascular fibroblasts (PF), whereas in penetrating spinal cord injuries, they mainly arise from meningeal fibroblasts (MF).

However, certain studies have suggested that in both penetrating and non-penetrating spinal cord injuries, myofibroblasts in fibrotic scars predominantly originate from GLAST+ type A pericytes.

Prior research demonstrated that the complete removal of fibrous scars often results in a large cavity and is not conducive to injury repair. Conversely, a partial reduction of scars can enhance axon regeneration and functional recovery, suggesting the existence of heterogeneity in the function of fibrous scars following SCI.

“Given that PDFGRβ is expressed in both pericytes and fibroblasts, we used PDFGRβ-CreER::R26-TdTomato transgenic mice to enrich PDFGRβ+ cells before and after SCI,” said Professor Dai.

ScRNA-seq analysis revealed that PDFGRβ+ cells encompassed pericytes/vSMCs and fibroblasts, with the fibroblasts transforming into myofibroblasts after SCI.

The team employed Col1a2-CreER::R26-TdT mice to label fibroblasts, NG2-CreER::R26-TdT, and Myh11-CreER::R26 mice to track the cell fate of pericytes/vSMCs.

Their results further corroborated the contribution of fibroblasts to fibrotic scar formation and ruled out the contribution of pericytes/vSMCs.

Fibroblasts were subsequently classified into Crabp2/Emb+ meningeal fibroblasts (CA-F) and Lama1/Lama2+ pia/perivascular fibroblasts (LA-F) based on the defined markers. Immunostaining indicated that LA-F was located in the parenchymal perivascular space and the meninges inside of CE-F in the uninjured spinal cord.

After either transection or crush SCI, CE-F was distributed in the core of the fibrotic scar, surrounded by LA-F. Lineage tracing using Crabp2-CreER::R26-TdT mice confirmed the heterogeneous distribution of LA-F and MF-derived CE-F following SCI. CA-F expressed elevated levels of cholesterol synthesis-related genes and extracellular matrix genes Col1a1 and Fn1, while LA-F highly expressed extracellular matrix genes such as Col4a1 and Lama1.

This phenomenon was conserved between mice and monkeys.

Additionally, scRNA-seq analysis and in vitro experiments demonstrated that LA-F was implicated in lipid transport and angiogenesis.

The study clarified the heterogeneity in the cellular composition, origin, distribution, and function of fibrotic scars after spinal cord injury, providing answers to longstanding scientific inquiries in this field and establishing a theoretical foundation for the specific regulation of fibrotic scars.